Age-Induced Reprogramming of the Transcriptional, Signaling, and Metabolic Landscapes in Murine Bone Marrow Mesenchymal Stem Cells

Researcher(s)

  • Aarushi Patel, Biological Sciences, University of Delaware

Faculty Mentor(s)

  • Anja Nohe, Biological Sciences, University of Delaware

Abstract

Aging significantly impacts bone health, contributing to a higher risk of osteoporosis or bone fragility. Osteoporosis occurs when osteoclast activity becomes excessive or unregulated, leading to increased bone breakdown that outpaces the bone formation by osteoblasts. Aging plays a critical role, as it leads to cellular changes that cause this imbalance to occur. This study compares the proteomic data of 6-month-old and 15-month-old mice to determine key proteins that play a regulatory role associated with aging in bone tissue. This process began with the filtering of the original mass list for relevance to then comparing up- and down-regulated protein lists to known and peer-reviewed databases to search for multifunctional and highly-connected proteins. Proteomic analysis identified a subset of differentially expressed proteins, from which three key genes were prioritized based on their interconnectedness within a protein network and multifunctional roles in aging-related pathways.