Researcher(s)
- Adia Jones, Plant Science, University of Delaware
Faculty Mentor(s)
- Harsh Bais, Plant & Soil Science, University of Delaware
Abstract
The increasing demand for sustainable agricultural practices has driven interest in biological alternatives that improve soil health, crop quality, and nutritional value. Ergothioneine (EGT), a naturally occurring amino acid with potent antioxidant, anti-inflammatory, and antimicrobial properties, has recently gained attention for its potential health benefits. Although EGT cannot be synthesized by plants or humans, it is produced by certain microbes, including an actinobacteria species, Streptomyces coelicolor. This study investigates whether EGT can be taken up by plants and whether microbial interactions can enhance this uptake. Using Arabidopsis thaliana as a model plant system, we applied EGT through three different treatments: direct ergothioneine inoculation, co-application with other synthetic communities (SynCom) of plant growth-promoting rhizobacteria (PGPR), and co-application with Streptomyces coelicolor. Microbial cultures were prepared and verified through standard assays, and sterile plant inoculations were performed under controlled conditions. Ergothioneine uptake was quantified using ultra-performance liquid chromatography (UPLC). Confocal microscopy staining and imaging confirmed root colonization of A. thaliana by S. coelicolor, supporting its potential role as a root endophyte. Preliminary results suggest that SynCom presence with S. coelicolor may enhance EGT uptake, indicating a possible route for microbial-assisted biofortification. This research contributes to a broader understanding of plant-microbe interactions and offers promising insight into naturally enhancing plant nutritional profiles for human benefit.